Abstract

Growth, acid production, bile tolerance and adherence to columnar epithelial cells of four species of bifidobacteria.

Author(s): Ahmed Zahran

Four types of bifidobacteria were read for development, corrosive creation; bile acids resistance and adherence to sheep epithelial cells were studied. The species examined were Bifidobacterium longum ATCC 15707, B. bifidum ATCC 2203, B. angulatum ATCC 2238 and B. breve ATCC 2258 were tested for growth and environmental conditions. The log phase of B.longum and B.angulatum were found to plan 14 hours phase whereas the log phase of the opposite two species were found to plan 17 hours of inoculation. Both B. longum and B. angulatum reduced the pH faster than both B. bifidum and B. breve. B. longum had the very best growth and B. breve had rock bottom rate of growth. All the studied species exhibited a point of bile tolerance. B. longum and B. bifium were more immune to bile acids than the opposite two species. Adhesion of the four species to the columnar epithelial cells of the tiny intestine of sheep was studied.All the tested species showed some degree of adhesion; however,